How to correlate genetic, cytological and physical maps?
A Cloned Gene or STS is the key. (Remember, any time you have an STS, you can always use PCR to make lots of that DNA.), See Fig. 4.35 for an integrated genetic, physical and cytological map of human chromosome 1. A. Physical maps are often restriction maps produced by overlapping cloned DNA fragments or Southern blotting using cloned DNAs as the hybridization probe. (See also Fig. 4.39) B. Genetic maps are produced using RFLPs deduced from Southern blots using cloned DNA as the probe or STSs using DNA sequence information derived by sequencing a cloned DNA fragment. C. Cytological maps are derived using in situ hybridization, again using a cloned DNA as the hybridization probe. XI. The utimate physical map is the complete sequence of the genome. (pp.
