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For example, if you have 60500 pmoles (60.5 nmoles) of primer, you can add 60.5 µl of dH20 to make a 1mM stock solution of primer (60.5 nmoles + 60.5 µl of dH20 = 1 mM solution of primer). Dilute 1:100 = 10 µM If you use 1 µl of 10 µM primer in 25 µl PCR reaction, your final primer concentration will be 0.5 µM.
