How to clean glassware to eliminate contaminants for LCMS?
Dear all, I am a LCMS beginner and I found that contamination is big problem when working with LCMS for picogam level. My lab have worked with HPLC-UV and fluorescent detector, we already have a SOP for cleaning glassware by soap only and we have no problem but this procedure does not work for glassware of LCMS: after cleaning, we try to test by putting mobile phase into extraction tube and shaking and injecting the resultant solution, we can detect a peak of analyte at level of LLOQ, and with that “dirty” glassware, the precision and accuracy of the method can not meet the requirement. Now I am trying to write a procedure to clean LCMS glassware to eliminate or minimize the contaminants. Could any one please give me some experience of cleaning glassware for LCMS? Thank you very much in advance!
