How to calculate amount of m grams of oligomer I have?
There is more than one algorithm that can be applied to determine the precise extinction coefficient for any specific oligonucleotide sequence of DNA. To avoid repeated debates over the nature of mass vs. absorbance, we report our yield in ODU and let each person decide what to multiply by according to there own conscience. There are many computer programs available, which will conjure up a number, or those of you who are less detail oriented use 33 ug/ A260U.
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