How to adhere the slice on the MED probe?
Use the slice anchor. Please refer to MED64 Procedure. After putting the slice onto the MED probe, upon stimulation I can not see any responses. This is usually due to the slice not being fully adhered on the electrodes particularly at the beginning of an experiment. Make sure to use a slice anchor. Even with the weight, it might take some time for a slice to adhere to the electrodes after starting perfusion. You might need a larger stimulus current to acquire evoked signals at the beginning of an experiment. After putting the slice onto the probe, upon stimulation all I see are large capacitance currents in many or all of the electrodes. In addition, during recording, clear EPSP signals continuously fade in and out. What is the cause of this inconsistency? This is usually due to the slice not being fully adhered to the probe. Using a weight should get rid of this problem.
