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How the MTT solution is prepared for viability assay?

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How the MTT solution is prepared for viability assay?

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A stock solution of MTT powder (Sigma Cat No, M5655) at 5 mg/ml is prepared in PBS, filtered and stored at -20C in 1 ml samples. The day of the experiment, a sample is diluted at 10% in SkinEthic Maintenance medium.

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