How should samples be processed before using the StellARray™ Gene Expression System?
High quality RNA is ESSENTIAL for obtaining accurate gene expression results. The most important prerequisite for any gene expression analysis experiment is consistent, high-quality RNA from every experimental sample. Therefore, the sample handling and RNA isolation procedures are critical to the success of the experiment. Residual traces of proteins, salts and especially genomic DNA will negatively impact the accuracy of gene expression experiments. The use of high quality products for RNA purification is strongly recommended. A DNase treatment step (on-column or in separate tube) must be performed. For CNV analysis, we recommend using a DNA extraction and purification kit capable of producing high quality DNA.
