How should plant samples be prepared for analysis?
Plant Processing Facilities are located at the ground floor level of the Kenzo Hemmi Bldg. All requests for the use of these facilities must be e-mailed to l.molina@cgiar.org, with cc to a.boncajes@cgiar.org at least one week before the date of use. Refer to the Guidelines in using ASL Plant Processing Facilities and consult a.boncajes@cgiar.org for any assistance needed. 1. For Kjeldahl N analysis, macro- and micronutrient analysis by acid digestion and ICP-OES (sample size: 0.841 mm, No. 20 mesh) Oven-dry samples at 80oC. Large dried samples are first ground using stainless steel beater cross grinder and subsampled by quartering to a manageable size. To obtain homogeneous powder, samples are finely ground using stainless steel cyclone Udy Mill or Wiley Mill and allowed to pass through a 20-mesh stainless steel sieve to avoid metallic contamination. After grinding, the whole sample must be mixed thoroughly. In between samples, the mill is thoroughly cleansed with a stiff-bristled brus
