How should I scale up AquaPlasmid and companion solutions?
You may use AquaPlasmid miniprep protocol as a reference. For each milliliter of culture, you use 50 ul of deionized water to resuspend the cells, 30 ul of AquaLysis Solution to lyse the cells, 30 ul of AquaPlasmid Solution to extract plasmid DNA and precipitate cell debris, and then 30 ul of AquaPlasmid Solution to precipitate plasmid DNA from 100 ul of cleared cell lysate. 8. Should I use centrifugation to rinse the DNA pellet? I am afraid that my pellet may lose during ethanol rinse. It is unnecessary. Gently shoot the ethanol solution into the tube along the sidewall, rotate it, and then flip the tube to discard the ethanol. Repeat the ethanol rinse 3 times. For midi and maxiprep ensure that the DNA pellet remains attached at the bottom of the tube before discarding the ethanol solution. For miniprep, the DNA pellet may be invisible.
