How should I scale up AquaPlasmid and Aqualysis Solutions?
A.: You may use AquaPlasmid miniprep protocol as a reference. For each milliliter of culture, you use 50 ml of deionized water to resuspend the cells, 30 ul of AquaLysis Solution to lyse the cells, 30 ul of AquaPlasmid Solution to extract plasmid DNA and precipitate cell debris, and then 30 ul of AquaPlasmid Solution to precipitate plasmid DNA from 100 ul of cleared cell lysate.
