How should I resuspend my oligos?
Eurofins MWG Operon oligos are always shipped salt-free. Before opening, spin the tube at 1000 rpm for 5 min to ensure that the oligonucleotides are at the bottom of the tube. It is recommended that the oligonucleotides should be resuspended in a sterile buffered solution (e.g., TE at pH 7.0). Please vortex your oligonucleotides thoroughly after resuspension. The oligonucleotides may not readily dissolve in sterile, distilled water. If NaOH is added to the water, the pH will rise to 7.0 and this should help. If the oligonucleotides are resuspended at pH <7.0 (deionized water may have a pH as low as 5.0), the oligonucleotide could begin to degrade and may lose functionality within a couple of weeks. For optimal long-term storage, it is recommended that the oligonucleotides should be stored dry at -20°C in the dark. If numerous experiments are planned using the same oligonucleotide, prepare aliquots, dry them and store the aliquots at -20°C. Use clean, sterile labware for all transfers.
