How should I quantitate my DNA before GoldenGate genotyping?
Illumina recommends using a method of direct quantitation specific to double-stranded DNA (dsDNA), such as with the PicoGreen reagent. Alternative indirect methods such as UV spectrometry/NanoDrop can incorrectly report the concentration of dsDNA in your sample when single-stranded DNA, oligonucleotides, RNA, and/or proteins carried over from DNA extraction are present in solution.
