How should I qualify my cDNA for use with the WT-Ovation™ Exon Module?
You must use cDNA generated with a validated NuGEN Amplification System product. The concentration of starting cDNA must be determined to ensure adequate input into the ST-cDNA reaction. You may chose to further qualify the starting cDNA by performing qPCR assays as recommended in the appropriate NuGEN Amplification System user guides.
