How should I qualify my cDNA for use with the FL-Ovation™ cDNA Biotin Module V2?
You must use cDNA generated with a validated NuGEN Amplification System product. The concentration of starting cDNA must be determined to ensure adequate input into the F&L reaction and therefore onto the arrays, please see Table 2 in the Protocol section of the FL-Ovation™ cDNA Biotin Module V2 user guide for cDNA input requirements. You may chose to further qualify the starting cDNA by performing qPCR assays as recommended in the appropriate NuGEN Amplification System user guides.
