How should I prepare 20 mM citrate, pH 3.0?
To prepare 20 mM Citrate Buffer, pH 3.0, dissolve the appropriate amount of citric acid in deionized H2O, adjust the pH to 3.0 with NaOH, and sterile-filter through 0.2 µM filter. For example, for 100 ml, dissolve 0.384 g citric acid (anhydrous) in 90 ml of deionized H2O, then adjust pH with a few drops of 5M NaOH. Once pH is 3.0, bring the volume up to 100 ml with DI water.
