How should I physically organise my samples and/or primers when I send them to the Sequencing Service?
If the samples and/or the primers are submitted in PCR strip tubes, the best way to organise them is to regroup all samples (plasmids or PCR products) one after the other. The tubes must be properly labelled with the corresponding well ID on the sample submission form. Example: A01, A02, A03… For the primers, it is the same idea; they must be aliquoted in PCR strip tubes in the same order than the samples are placed. If the samples and/or the primers are submitted in a 96-well plate, the best way to organise them is to regroup all samples (plasmids or PCR products) one after the other starting at well A01 and following the plate order which is A01 to A12, B01 to B12 and so on. The primers can be aliquoted on the same plate in the lower rows if there is enough space or in another plate properly identified.
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