How should I handle the product (GenPoolTM ORF clones)?
Products of the human genes in GenScript pDream2.1/MCS vector are delivered as lyophilized powder, and they can be stored forever at -20C. You can handle the products in the same way as you handle regular DNA plasmid. Before use, add 20 l water to dissolve it. Vortex diligently and make sure the DNA is dissolved. If needed, incubate at 50C for 10 min. You can use it directly if you only need less than a few g of DNA. For large amount of DNA, take 1 or 2 l of the solution and transform competent DH5α or TOP10 cells for a MidiPrep or MaxiPrep using DNA purification kits. • Are the genes sequencing verified? A: Yes. The sequences of genes are confirmed by DNA sequencing. • Do you guarantee expression? A: We have tested cGFP (pDream2.1-cGFP) expression in E. coli, Sf9 cells and mammalian cells. pDream2.1-cGFP vector (GenScript Catalog No.: SD0220) is also available as a positive control. Because each gene has different property, we do not guarantee that the ORF you purchased will express i
