How should I handle the fresh hepatocytes once I receive them?
Upon receipt, fresh hepatocytes in suspension should be centrifuged at 4ºC immediately, following our instructions. Plated hepatocytes should be quickly removed from the box and the shipping medium replaced with fresh culture medium. The preservation medium used during shipment is meant to be kept cold; if the cells warm past 8ºC, the shipping medium begins to have a toxic effect on the hepatocytes. We recommend that you let your plated cells acclimate overnight before beginning your experiment.
