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How should I coat the plates with antibody? How should the plates be stored after coating?

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How should I coat the plates with antibody? How should the plates be stored after coating?

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Avoid air bubbles when coating the plates, as this may cause blank spots on the wells. Coated plates should be stored overnight at 4° C in a humidified box. To avoid contamination, maintain sterile techniques during the coating and incubation periods.

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