How should I clean my TSKgel SWxl column?
We recommend the following methods to clean a column. After each step wash the column with DI water to remove solvent, salt, or detergent. 1. Clean in the reverse flow direction (it will not harm the column) at half the maximum flow rate. 2. Use 20% acetonitrile to remove contaminants that are held onto the column by hydrophobic interaction. 3. Use 0.5M salt (NaCl, sodium sulfate) at pH 3 to remove positively charged contaminants that are bound to the column by ionic interaction, such as with residual negatively charged silanol groups. 4. If the above steps do not work, use 6M urea or guanidine hydrochloride.
