How sensitive is the PhosphorImager system?
A. It’s about ten times more sensitive than film exposed without fluors or intensifying screens. For 14C, 2 dpm/mm2/hour will give a signal that is at least twice background. This assumes that the 14C is not buried in an absorbing or deflecting material such as a thick TLC silica matrix. This level of sensitivity is easy to achieve using 14C-glucose spotted on Whatman filter paper or nitrocellulose paper. Storage phosphor is not very sensitive to tritium-labeled molecules in gels, since the gel matrix prevents the weak emissions from reaching the screen. It’s better to use a fluor, such as Amersham AmplifyTM, and a low temperature exposure to sensitive film for this application. However, PhosphorImager systems work well for tritiated whole-body-autoradiography slices, because tritium is right on the surface of the sample. Special tritium screens are required for this application.
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