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HOW REPRODUCIBLE ARE CUSTOMARRAY CHIPS?

April 26, 2017chips customarray reproducible

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HOW REPRODUCIBLE ARE CUSTOMARRAY CHIPS?

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We hybridized 20 identical CustomArray chips with the same RNA target sample prepared from the Universal Human Reference RNA (Stratagene) and labeled with Cy3 and Cy5 according to the dual-color scheme. Our raw data (no background correction) showed the following average correlation coefficients among different pairs of 20 chips: 0.97 for the Cy3 color channel, and 0.98 for the Cy5 color channel. In total, 97% of all pair-wise correlation coefficients were over 0.95.