HOW REPRODUCIBLE ARE COMBIMATRIX MICROARRAYS AFTER STRIPPING AND RE-HYBRIDIZATION?
CombiMatrix hybridized several sectored CustomArray™ 4x2K chips with the same labeled RNA target sample prepared from the Universal Human Reference RNA (Stratagene). Then the microarrays underwent 3 stripping and re-hybridization steps with the same labeled target sample. The raw data (without normalization and background correction) were log-transformed, and pair-wise correlation coefficients (r2) were calculated for subsequent re-hybridizations. The average r2 was 0.91. In total, approximately 80% of all correlation coefficients exceeded 0.9. Scatter plots of log-transformed data are shown in Fig. 3 demonstrating high consistency between four subsequent hybridizations. Please note that the 5th, additional hybridization of the same microarray results in a decrease in data quality (the bottom panel, right plot, comparison of 1st hybridization with the 5th one for the same sector).
