How ProtoArray® technology identify autoantibodies?
Steps • Samples are obtained from control and diseased or treated patients. • ProtoArray® Human Protein microarray are blocked and incubated with diluted samples. Autoantibody biomarkers are visualized with a fluorescent dye-labeled secondary antibody. IgA, IgG and IgM subtypes can be detected on ProtoArray® microarrays with the proper secondary antibody. • Data analysis with the free software tool ProtoArray® Prospector enables identification of antigens recognized by elevated autoantibodies in a patient’s sample.