How precise is SBS?
SBS is a digital method of analyzing gene expression or small RNAs; in other words, it is based on a direct count of sequences from a given cDNA library, using sequence tags to determine the abundance of cognate transcripts in the library. In digital expression analyses, comparisons between large libraries facilitate the detection of significant differential expression for genes expressed at low levels. It is far better at detecting low levels of transcripts than RNA gel blots, simply because it’s possible to sequence so deeply.
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