Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.
Depending on which service, Affymetrix, Illumina, spotted arrays require at least 2ug, 500ng and 1ug respectively, in RNase-free water, with concentration greater than 100ng/ul. Bioanalyzer or OD check needs about 50ng in 2ul. Extra amount is always helpful, especially when first trial is failed, although the chance is small.
