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How much time should I wait for the Antigen/ Antibody to bind on the membrane before an assay is run?

April 26, 2017Antibody antigen assay bind membrane run time wait

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How much time should I wait for the Antigen/ Antibody to bind on the membrane before an assay is run?

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The binding of the protein to the NC membrane grows stronger as it is dehydrated more and more. Usually, at room temp, (20-30 C), 50-60% RH the binding will reach a maximum in 3-5 days. For development, reasonable results can be expected with overnight drying at room temperature.