How much time does it take to complete the amplification reaction?
Amplification reactions of typical sized M13 or plasmids (up to 12 kb) are completed in 4-6 h with the TempliPhi 100 or 500 Amplification Kits (25-6400-10 & 25-6400-50) or 16-18 h with the TempliPhi DNA Sequencing Template Amplification Kit (25-6400-01). Amplification of large templates (fosmids, BACs) is completed in 16-18 h with TempliPhi 100 or 500 Amplification Kits.
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