HOW MUCH STARTING MATERIAL (RNA OR DNA) DO I NEED FOR AN ASSAY WITH CUSTOMARRAY CHIPS?
CustomArray chips have been shown to work with a wide variety of labeled target nucleic acids produced using different commercial kits. So the amount of initial material that you need to start with depends on the applied technique for target amplification and labeling. For example, T7 polymerase-based RNA amplification kits (e.g. from Arcturus and Ambion) result in very high degrees of amplification (especially when used in double rounds). Thus you may start with as little as 100 ng of total RNA and still get enough material to hybridize with CustomArray chips.
