How much ST-cDNA yield can I expect?
Q9. What is the size range of ST-cDNA generated by the WT-Ovation™ Exon Module? As measured with an Agilent Bioanalyzer, the ST-cDNA is shorter than the template cDNA. Q10. Has NuGEN performed reproducibility studies on the WT-Ovation™ Exon Module? Yes, our studies have included sample to sample, lot-to-lot, and operator-to-operator reproducibility. See WT-Ovation™ Exon Module Technical Report #1 for some of these studies. Q12. Should I purify the input cDNA before ST-cDNA generation? Yes. The protocol requires a specific quantity of amplified cDNA and the cDNA must be purified in order to be accurately quantitated. Q13. What are the recommended storage conditions for the ST-cDNA? The ST-cDNA may be stored at -20°C. Ensure the vials are well sealed and avoid multiple freeze thaw cycles. Q14. What types of arrays work with the WT-Ovation™ Exon Module cDNA? The WT-Ovation™ Exon Module has been validated to generate cDNA targets ready for fragmentation and labeling with the FL-Ovation cDN
