How much sample will I lose in the Slide-A-Lyzer Cassette?
The Slide-A-Lyzer Cassette membrane is made of the same regenerated cellulose that is used in dialysis tubing, so a researcher should expect about the same protein loss one would experience with tubing. As a guide, a 1 mg/ml solution will generally have a recovery rate of greater than 95%. At 100µg/ml, the recovery rate drops to 75%-80%. With a dilute (e.g., 10 µg/ml) protein solution, one can lose up to 50% or more of the sample.
