How much RNA is required for the RobusT™ RT-PCR Kit?
The minimum amount of RNA that can be amplified using the RobusT RT-PCR Kit is dependent on many factors such as primer design, target size, and abundance of message. For the Positive Control RNA provided, the minimum amount of RNA required is 1 fg. Excellent amplification results can be obtained using total RNA template levels in the range of 1 pg -1 µg per reaction, or poly(A)+ RNA template levels in the range of 1 pg -100 ng. This system was able to detect ARF (human ADP ribosylation factor I), a low abundance message from as little as 100 pg of human skeletal muscle total RNA.
