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How much REPLI-g amplified mitochondrial DNA should be used in a subsequent PCR reaction?

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How much REPLI-g amplified mitochondrial DNA should be used in a subsequent PCR reaction?

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The REPLI-g Mitochondrial DNA Kit amplifies mitochondrial DNA approximately 40 million-fold. The small size of the mitochondrial genome leads to very high copy numbers per microliter of amplified DNA….

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