How much protein do I need to identify a phosphorylation using IMAC purification?
Isolation of phosphopeptides by IMAC purification probably requires 10 pmoles or more phosphorylated protein. It depends on the phosphopeptide sequence, however. TiO2 is likely a far more sensitive method for isolation of phosphopeptides. We plan to provide TiO2 phosphopeptide purification as a service soon.
Related Questions
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- How much protein do I need to identify a phosphorylation using IMAC purification?
