How much protein can I load on to the Membrane Adsorber?
The following table highlights the maximum binding capacities using BSA as the standard protein for D and Q membrane and lysozyme as the standard protein for S and C membrane. 1 cm of Q and S membrane displays a minimum binding capacity of 0.8 mg/cm (i.e. 29 mg / ml membrane) 1 cm of D and C membrane displays a minimum binding capacity of 0.6 mg/cm (i.e. 22 mg / ml membrane) Actual yields depend on specific protein sample and selected pH and salt conditions. Yields established using 1 mg/ml BSA in 10 mM potassium phosphate buffer pH 7.0 with Sartobind Q and D and 1 mg/ml lysozyme in 10 mM sodium phosphate buffer pH 7.0 with Sartobind S and C.
