How much mRNA can be isolated with the µMACS™ mRNA Isolation Kits?
Important aspects concerning mRNA yield from biological samples are cell or tissue type, origin and status, and the intactness of the sample. Hence, immediate disruption of tissue or cells in lysis buffer, quick-freezing, or submersion of cells or tissue pieces in an RNA-stabilizer like PrepProtect™ Stabilization Buffer* is essential. Information about average RNA yields can be downloaded from www.miltenyibiotec.com. Depending on the source and quantity of the mRNA, there are three different µMACS™ mRNA Isolation Kits to choose from. Using the MACS® Technology, mRNA can be isolated with very high efficiency. This is particularly important when working with low amounts of starting material, such as rare cells or samples from microdissection, or when rare transcripts have to be isolated. The MicroBeads effectively label the mRNA molecules in a cell. Even mRNA from five Jurkat cells was successfully isolated using the µMACS mRNA Isolation Kit. * Submersion of tissue or cells in PrepProtec
