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How much labeled, fragmented cRNA must I send and at what concentration?

April 26, 2017concentration crna fragmented labeled send

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How much labeled, fragmented cRNA must I send and at what concentration?

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The amount of labeled, fragmented cRNA varies depending upon the type of array you are using. Test chips are MiniArrays, and most other arrays are Standard Arrays (see your chip packaging insert for this information). The final concentration in all cases should be 0.05µg/µL Micro/Mini Array: 5µg in no greater than 40µL Midi Array: 10µg in no greater than 80µL Standard Array: 15µg in no greater than 100µL While we currently do not require you to send us copies of gel photos to determine the quality and quantity of your sample, we strongly suggest that you follow the recommendations in the manual to confirm both sample quality and quantity.