How much Immobilized TCEP Disulfide Reducing Gel should I use?
In general, a volume of gel equal to 0.5-1.0 X the sample volume is sufficient to ensure timely and efficient sample reduction. For example, use 50-100 ml of packed Immobilized TCEP Gel for 100 ml of sample. Although it may be possible to use less Immobilized TCEP, using too little will compromise speed and/or completeness of reduction.
