How much Extracel should I use in my tissue culture dishes?
The amount of hydrogel you use is up to you and your application. However, to culture cells normally on the surface of an Extracel hydrogel we routinely use a gel thickness of ~1 millimeter. If you want to make thin films, add a greater volume of hydrogel to each well. Rock the plate to cover the entire well and then pipette out the excess hydrogel. Please refer to our Technical page for more information on how to coat plates with Extracel.