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How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System?

April 26, 2017cDNA detection mirna miscript PCR real-time sufficient system transcripts

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How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System?

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For detecting miRNA generated by the miScript System, 0.5 ng–1 ng cDNA per PCR is sufficient. Therefore cDNA made with 1 µg input RNA is usually adequate to perform miRNA profiling using all avai…