How much can I dilute Bactosomes in my assay?
The QC for all batches of Bactosomes incorporates an assay for linearity of substrate turnover with P450. The upper limit for this is given in the accompanying Data sheet. Graphs showing typical values for the lower end of this assay are available on the product sheets on our website and in our catalogues. We have found no artefacts associated with the dilution of Bactosomes in assays however, we recommend diluting the Bactosomes at the last minute before adding them to your assay as the diluted enzyme (particularly dilutions greater than 1/10) is less stable in the absence of substrate.
