How much antigen is needed to make good titer antibodies?
Amounts of antigen needed to produce good titer antibodies obviously depend upon the antigenicity of a given “antigen” and host species. Bacterial/viral antigens are very often highly immunogenic than mammalian proteins. In our experience, the antigen amounts given below will normally suffice to make good titer antibodies. These estimates are for using up to 2 animals using our std. protocols.- Purified and recombinant proteins: 500 ug – 2 mg. – Peptide-Protein Conjugates: 2 – 5 mg. – Proteins in SDS-PAGE gels: A band that is clearly visible upon staining (500 ug – 2 mg). – Proteins as precipitates: 500 ug – 2 mg.
