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How many Western blots can one do with 100 ug total protein in a lysate?

blots lysate protein total Western
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How many Western blots can one do with 100 ug total protein in a lysate?

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100 µg lysate is sufficient for 20~50 reactions. Depending on over-expression level and antibody affinity, some lysates can go as low as 0.1µg per load.

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