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How many times can the membrane be regenerated and reprobed with antibody?

April 26, 2017Antibody membrane regenerated reprobed times

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How many times can the membrane be regenerated and reprobed with antibody?

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The membrane can be regenerated up to five times. Forceps should always be used when handling the membrane, as the membrane becomes fragile when wet. The integrity of the peptides on the membrane is not altered by the regeneration protocol.