How many times can the affinity column be used?
• Although the capacity does decrease slightly with each use, the column can often be used for at least 10 additional purifications. We recommend storing the column at 4o C (avoid freezing or drying) and with PBS azide (added by default). In addition, it can help to filter the serum through a membrane (.45) before running it through the column (to help prevent the build up of particulates in the column). The column should also be washed between uses to remove any protein.
