How many first derivative peaks are displayed on the melt graph?
Multiple first derivative peaks indicate that multiple products were amplified during the PCR. Generally, primer-dimers melt before specific products. Refer to Smart Note 6.4 Intercalating Dye Assay on the Smart Cycler® System for more details on reducing primer-dimer formation. To change the number of melt peaks that are displayed on the graph or to change the qualifying height for the first derivative peaks, click Setup menu>System Defaults>Melt Settings. If the melt curve appears noisy (jagged), try smoothing the melt curve by averaging 3 –5 points. Click the Setup menu>System Defaults>Melt Settings.
