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How many copies of pKLAC1 can be tandemly integrated?

April 26, 2017copies Integrated pklac1

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How many copies of pKLAC1 can be tandemly integrated?

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The majority of multiply integrated strains contain 2-5 integrated copies of pKLAC1. While higher copy number strains may be attainable, they will be significantly more rare within a given population of transformants due to the reduced probability of their formation and to mitotic instability.