How many colors can the ImageStream system image?
The ImageStream system’s CCD camera produces six images of each cell, typically darkfield, brightfield, and four fluorescent colors. If desired, a fifth fluorescence color can be imaged in place of brightfield. Each image is used to calculate over 80 parameters that quantitate morphology, fluorescence strength, and fluorescence distribution. A six-image assay therefore results in ~500 parameters per cell. The ImageStream is compatible with a wide variety of fluorescent dyes commonly used in both flow cytometry and microscopy.
