How many cells can I infect with the amount of lentivirus provided?
The amount of cells that can be infected depends upon the cell line being used. For HeLa, A549 and HEK293T cells we have used 4µL (1 x 106 TU/ml via p24 assay) for each well containing ~16,000 cells in a 96-well plate. Primary or other difficult cells may require more lentiviral supernatant, but even at a 10 µL level, it would give you enough for 20 x 96-well reactions. We suggest decreasing the number of cells plated to increase the multiplicity of infection (MOI) if necessary. Also, performing a limiting dilution titer on your cell line will give you the optimal amount of viral particles needed for each assay.
