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How many cells are needed to obtain 100 micrograms of protein?

April 26, 2017cells micrograms needed obtain protein

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How many cells are needed to obtain 100 micrograms of protein?

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The amount of protein present in cells may vary with cell type. We typically use 1 to 10 million cells to get approximately 1mg of protein; only 10uL of which containing less than 100ug of protein is used for labeling. Start with 5 million cells whenever possible.